Uji Toksisitas Ekstrak Kulit Batang Pulai Basung (ALSTONIA SPATULATA BL) Dengan Metode Brine Shrimp Lethality Test

Abstract

An investigation on the toxicity of pulai basung (Alstonia spatulata Bl)extract has been done using brine shrimp lethality test (BSLT)method. The extract was obtained by macerating the stem barks of A.spatulata using n-hexane and methanol respectively. The methanolextract was fractionated based on a method for isolation of alkaloidcompounds to afford a crude alkaloid extract. Since a trace ofalkaloids was detected in the remaining aqueous solution, this solutionwas then partitioned with n-butanol to give a n-butanol extract. Aneffect of toxicity from those extracts were identified for their deathpersentage of Artemia salina nauplii and counted for their LC50 byprobit analysis. The result showed that the crude alkaloid extract wasmore toxic than other extracts with LC50 163 ppm.